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X-ray Fluorescence Laboratory for Trace Element Detection in Human and Biological Samples

Researcher: Ana Pejović-Milić

The X-ray fluorescence laboratory for trace element detection in human and biological samples was established through investments by CFI /OIT and NSERC-Equipment Grants (P.I. A. Pejović-Milić). Presently the research is focused on the X-ray fluorescence elemental analysis, both in vivo and ex-vivo, of calcified tissues.

Work conducted in this research facility provides opportunity for both destructive and non- destructive analyses of any samples at detection limits of ppm and ppb levels. It houses an energy-dispersive, ppb detection resolution, X-ray fluorescence spectrometry (EDXRF, Bruker), a total reflections, ppm detection resolution, X-ray fluorescence spectrometry (TXRF, Bruker) and a hand held (mobile, ppm or ppb detection resolution) X-ray spectrometer (HHXRF, Tracer III, Bruker) available for the quantification of trace/toxic elements in biological tissue and/or other materials. Additional instrumentation that is available to Ryerson researchers and students include X-ray fluorescence components/equipment suitable for assembling different custom systems for non-destructive trace and toxic element analysis (Gamble Technologies SLP -16220-P: Silicon (Li) X-ray Detectors, a DSPEC-PLUS Digital Gamma-Ray Spectrometer with MAESTRO-32 Software, Canberra GL2020R HPGe Low Energy Photon Spectrometer, DSA-2000 Digital Gamma-Ray Spectrometer with Genie 2000 software). The world’s first in vivo X-ray fluorescence based diagnostic tool was developed in this laboratory, along with providing the ability to non-destructively measure many other elements, such as manganese, lead, gold, arsenic, selenium, silver and many others, accumulated in biological and/or other samples.

Equipment

S2 Ranger Energy-Dispersive X-ray spectrometer (Bruker-AXS, Madison, WI, USA)
S2 PicoFox Total Reflection X-ray spectrometer (Bruker-AXS, Madison, WI, USA)
Tracer III-SD Handheld spectrometer (Bruker-AXS, Madison, WI, USA)
Gamble Technologies SLP - 16220 - P: Silicon (Li) X-ray Detectors
DSPEC-PLUS Digital Gamma-Ray Spectrometer with MAESTRO-32 Software
Canberra GL2020R HPGe Low Energy Photon Spectrometer
DSA-2000 Digital Gamma-Ray Spectrometer with Genie 2000 software
Ball mill
Pellet press

Research Area: 
Trace Element Detection